Oral Supplementation with Herbal Phytohormones on the Reproductive Performance of Female Climbing Perch (Anabas testudineus)

Authors

  • Helmizuryani Helmizuryani Program Studi Akuakultur, Fakultas Pertanian, Universitas Muhammadiyah Palembang, Palembang, Sumatra Selatan, Indonesia
  • Muhammad Nizar Program Studi Akuakultur, Fakultas Pertanian, Universitas Muhammadiyah Palembang, Palembang, Sumatra Selatan, Indonesia
  • Muhammad Akbar Program Studi Akuakultur, Fakultas Pertanian, Universitas Muhammadiyah Palembang, Palembang, Sumatra Selatan, Indonesia

DOI:

https://doi.org/10.32502/jgsa.v6i2.2309

Keywords:

natural phytohormones, climbing perch fish, maturation of the parent gonad, reproductive performance

Abstract

Limited seed due to low reproductive performance of the parent is still an obstacle in the cultivation of betok fish (Anabas testudineus). The use of natural phytohormones based on herbal ingredients has the potential to be a safe and environmentally friendly alternative to increase fish reproduction. This research aims to evaluate the effectiveness of various herbal ingredients on the reproductive performance of betok fish and determine the best herbal ingredients based on the gonad maturity level (TKG) and gonad maturity index (IKG). The research was carried out in the aquaculture wet laboratory, Faculty of Agriculture, Muhammadiyah University, Palembang for 45 days with 4 treatments and 3 repetitions, P1: Treatment with turmeric extract, P2: Treatment with katuk leaf extract, P3: Treatment with papaya leaf extract and P4: Treatment with sprout/bean sprout extract. The research results showed that natural phytohormone supplementation based on herbal ingredients provided a positive response to the reproductive performance of betok fish (Anabas testudineus). Sprout/bean sprout extract is the best treatment in increasing growth, gonad maturity level, and gonad maturity index, while papaya leaf extract produces the highest fecundity. All treatments resulted in a 100% survival rate and did not reduce the water quality of the rearing media. Thus, herbal ingredients have the potential to be used as a natural alternative to increase the reproduction of betok fish in sustainable cultivation activities.

References

Amulejoye, F.D., Mitra, A., Hasimuna, O.J., et al. 2025. “Application of medicinal plants in fish reproduction for sustainable aquaculture practices: A review”. All Life 18(1). 2575767. https://doi.org/10.1080/26895293.2025.257576 7

Azwanida, N.N. 2015. “A review on the extraction methods use in medicinal plants, principle, strength and limitation”. Medicinal & Aromatic Plants 4(3). 196. https://doi.org/10.4172/2167-

1000196.

Citarasu, T. 2010. “Herbal biomedicines: a new opportunity for aquaculture industry”. Aquaculture International 18. 403–414. https://doi.org/10.1007/s10499-009-9253-7

Effendie, M. I. 2002. “Biologi Perikanan (Edisi revisi, cetakan ke-2)”. Yogyakarta: Yayasan Pustaka Nusatama.

Hamid. A.K.N, Somdarea, O.P, Harashida, M.A.K, et al. .2022. “Effect of papaya (Carica papaya) leaf extract as dietary growth promoter supplement in red hybrid tilapia (Oreochromis mossambicus Oreochromis niloticus) diet”. Saudi Journal of Biological Sciences (29). 3911–3917. ttps://doi.org/10.1016/j.sjbs.2022.03.004

Helmizuryani. 2013. “Analisis biologi reproduksi ikan betok (Anabas testudineus) dari perairan alami”. Jurnal Fiseries 2(1). 35 – 39.

Helmizuryani, H., Muslimin, B., dan Khotimah, K. 2018. “Reproduction performance of climbing perch (Anabas testudineus) F1 and F2 broodstock with different dietary supplementation”. Jurnal Akuakultur Indonesia 17(1). 61–67. https://doi.org/10.19027/jai.17.1.61-67.

Helmizuryani, Muslimin.B. Djumanto, Aminah, R. I. S., and Khotimah, K. 2020. “The gonadal maturation of climbing perch (Anabas testudineus) with dietary supplement add-on feed”. AACL Bioflux 13(2). 885–892. http://www.bioflux.com.ro/aacl

Lubzens, E., Young, G., Bobe, J., & Cerdà, J. 2010. “Oogenesis in teleosts: How fish eggs are formed”. General and Comparative Endocrinology 165(3). 367–389. https://doi.org/10.1016/j.ygcen.2009.05.022

National Research Council. 2011. “Nutrient Requirements of Fish and Shrimp”. Wasinghton: National Academies Press.

Nóbrega, R.H., Schulz, R.W., et al. 2022. “Fish spermatogonial stem cells and reproduction: Advances and applications in aquaculture”. Reviews in Aquaculture14. 1814–1840.

Rachmawati, Hutabarat, J, Samidjan, I., and Windarto, S. 2020. “Utilization of papain as feed additive in the fish feed on activity of digestive enzymes, contents of nutrient and minerals of Sangkuriang catfish (Clarias gariepinus var. Sangkuriang) Diana”. AACL Bioflux (13)5. 2738 – 2744.

Reading, J.B, Andersen, K.L, Ryu, W.Y, et al. 2018. “Oogenesis and Egg Quality in Finfish: Yolk Formation and Other Factors Influencing Female Fertility”. Fishes 3(4). 45. https://doi.org/10.3390/fishes3040045

Pimpimol, T., Klahan, R., adn Chitmanat, C. 2018. “The effects of garlic, banana, and onion as prebiotic supplementation on growth performances, feed utilization, and survival rate of Anabas testudineus”. Asia-Pacific Journal of Science and Technology 23(4). 1–6. https://doi.org/10.14456/apst.2018.20

Sepehrfar, D., Yousefi, M., Sudagar, M., et al. 2023. “Role of phytochemicals in farmed fish reproductive performance: A review”. Iranian Journal of Fisheries Sciences 22. 1040–1068. https://doi.org/10.22092/IJFS.2023.130580

Xue, Z., Wang, C., Zhai, L., et al. 2016. “Bioactive compounds and antioxidant activity of mung bean (Vigna radiata L.), soybean (Glycine max L.) and black bean (Phaseolus vulgaris L.) during the germination process”. Czech Journal of Food Sciences 34(1). 68–78. https://doi.org/10.17221/434/2015-CJFS

Schulz, R. W., de França, L. R., Lareyre, J. J.et al. 2010. “Spermatogenesis in fish”. General and Comparative Endocrinology 165(3). 390–411.https://doi.org/10.1016/j.ygcen.2009.02.013

Downloads

Published

19-08-2026

Issue

Section

Articles

How to Cite

Oral Supplementation with Herbal Phytohormones on the Reproductive Performance of Female Climbing Perch (Anabas testudineus). (2026). Journal of Global Sustainable Agriculture, 6(2), 194-200. https://doi.org/10.32502/jgsa.v6i2.2309

Similar Articles

1-10 of 27

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)