Effectiveness of Dormancy-Breaking Treatments on the Germination of Indigofera zollingeriana
DOI:
https://doi.org/10.32502/jgsa.v5i2.862Keywords:
Indigofera zollingeriana, dormancy breaking, seed germination, scarificationAbstract
The availability of high-quality forage remains a major challenge in ruminant livestock systems, particularly during the dry season. Indigofera zollingeriana is a promising leguminous species with high protein content, yet its cultivation is constrained by high seed dormancy. This study aimed to evaluate the effectiveness of various dormancy-breaking treatments on the germination of I. zollingeriana seeds, both in general (across treatment groups) and specifically (to determine the most effective treatment). The experiment was arranged in a completely randomized design (CRD) with 17 non-factorial treatments and three replications, using two germination test methods: Top of Paper (TOP) and Between Paper (BP). Data were analyzed using ANOVA and orthogonal contrast tests at 5% and 1% significance levels. Results showed that all treatments (K1–K16) significantly increased germination percentage compared to the control, with averages of 49.88% vs. 36.00% (TOP) and 53.69% vs. 41.00% (BP). The most effective treatment was immersion in 95% sulfuric acid (H₂SO₄) for 10 minutes, which achieved the highest germination rate (88%) and the lowest dormancy intensity (0%), significantly outperforming physical, biological, and hormonal treatments. These findings indicate that short-duration chemical scarification can serve as a standard treatment for large-scale I. zollingeriana seed production. Further studies are needed to evaluate the physiological safety of the treatment and its impact on early seedling growth in field conditions.
References
Fahmi, Z.I., Purwanto, E., and Purnomo, D. 2020. “Hot water and sulfuric acid solution for increasing the viability and vigor of Indigofera zollingeriana”. IOP Conference Series: Earth and Environmental Science, Vol. 485 No. 1, 012099. https://doi.org/10.1088/1755-1315/485/1/012099
Ghahfarokhi, M.G., and Afshari, R.T. 2007. “Effects of different treatments on breaking seed dormancy and germination of Ferula gummosa”. Research Journal of Biological Sciences, Vol. 2 No. 4 pp. 498-502.
Ghahfarokhi, M.S., and Afshari, R.T. 2007. “Improving seed germination of Ferula gumosa using GA₃ and stratification”. Seed Science and Technology, Vol. 35 No. 1 pp. 76-80.
Haroni, A.R., Naim, M., dan Aini, N. 2015. “Pengaruh perlakuan KNO₃ terhadap perkecambahan benih Brassica juncea L”. Jurnal Agroteknosains, Vol. 3 No. 1 hal. 24-30.
Koobonye, B.K., Kwerepe, B.C., and Legwaila, G.M. 2018. “Effects of hot water and sulphuric acid scarification on seed germination of Leucaena leucocephala”. International Journal of Agronomy and Agricultural Research, Vol. 1 No. 4 pp. 1-9.
Koobonye, B.M., Batisani, N., and Malope, P. 2018. “Breaking dormancy in Leucaena leucocephala seeds using hot water treatment: Implications for rangeland rehabilitation”. African Journal of Range dan Forage Science, Vol. 35 No. 3-4 pp. 221-226. https://doi.org/10.2989/10220119.2018.1523694
Kunz, M., Wall, W.A., and Hohmann, M.G. 2020. “The role of fruits and fires in the germination of a rare subshrub, Amorpha georgiana (Fabaceae)”. Journal of the Torrey Botanical Society, Vol. 147 No. 1 pp. 1-9. https://doi.org/10.3159/TORREY-D-19-00016.1
Marsetyo, M., Wahyuni, N., Tarsono, T. and Pamulu, M. 2023. “The effect of scarification with different levels of sulfuric acid on seed germination and seedling growth of Indigofera zollingeriana Miq”. AIP Conference Proceedings, Vol. 2759 No. 1, 040007. https://doi.org/10.1063/5.0147785
Ministry of Forestry and Statistic Center Agencies. 2005. “Profil sosial ekonomi budaya masyarakat di kawasan hutan 2004”. Jakarta: Forestry Planning and Statistic Center, Ministry of Forestry and Directorate of Agriculture Statistic, Statistic Center Agencies.
Musa, Y., Sjahril, R., Nadir, M., and Syahril, W. 2023. “Germination growth of mutant (M2) Indigofera zollingeriana induced by gamma ray irradiation”. AIP Conference Proceedings, Vol. 2762 No.1, 030013. https://doi.org/10.1063/5.0150324
Nasri, N., Khaldi, A., Khouja, M. L., Touati, M. and Triki, S. 2013. “Effect of sulfuric acid pretreatment on dormancy breaking and germination of Prosopis juliflora and Dodonaea viscosa seeds”. African Journal of Biotechnology, Vol. 12 No. 14 pp. 1670-1675. https://doi.org/10.5897/AJB12.2911
Nasri, N., Khaldi, A., Triki, S. and Smaoui, A. 2013. “Breaking seed dormancy of Prosopis juliflora and Dodonaea viscosa: Implications for seedling production”. International Journal of Agriculture and Biology, Vol. 15 No. 4 pp. 696-700.
Pound, L.M., Ainsley, P.J. and Facelli, J.M. 2014. “Dormancy-breaking and germination requirements for seeds of Acacia papyrocarpa, Acacia oswaldii and Senna artemisioides ssp. × coriacea”. Australian Journal of Botany, Vol. 62 No.1 pp. 44-55. https://doi.org/10.1071/BT13155
Rajak, D.R., Bhardwaj, S.D. and Kumar, A. 2019. “Influence of potassium nitrate (KNO₃) on seed germination and seedling growth in Tectona grandis (L.) in nursery”. Journal of Pharmacognosy and Phytochemistry, Vol. 8 No. 1 pp. 312-315.
Rasyid, B., Hendra, J. dan Aziz, T. 2020. “Strategi penyediaan pakan hijauan pada musim kemarau di lahan kering”. Jurnal Peternakan Terpadu, Vol. 8 No. 2 hal. 100-107.
Somrug, K., Hemwong, S. and Jorjong, S. 2021. “Effect of seed dormancy breaking treatment and storability on seed quality of indigo (Indigofera tinctoria L.)”. Asian Journal of Plant Sciences, Vol. 20 No. 1 pp. 150-156. https://doi.org/10.3923/ajps.2021.150.156
Sutarman, S., Ginting, N.M., dan Widyantari, I.N. 2024. “Komparasi kelayakan usahatani padi sistem tanam pindah dan sistem tanam benih langsung di Kampung Yaba Maru Distrik Tanah Miring Kabupaten Merauke, Provinsi Papua Selatan”. Journal of Global Sustainable Agriculture, Vol. 4 No. 2 hal. 131-136. https://doi.org/10.32502/jgsa.v4i2.8178
Wina, E., Tangendjaja, B. and Atmomarsono, U. 2019. “Nutritional values and utilization of Indigofera zollingeriana as feed for livestock”. Jurnal Ilmu Ternak dan Veteriner, Vol. 24 No. 1 hal. 22-30.
Zeng, F., Leng, L., Bao, W.-K. and dan Zhou, Z.-Q. 2009. “Seed dormancy and germination of xeric shrub Indigofera lenticellata”. Chinese Journal of Ecology, Vol. 28 2 pp. 297-303.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Journal of Global Sustainable Agriculture

This work is licensed under a Creative Commons Attribution 4.0 International License.











